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multiple sequence alignment
The
WHAT CAN MSA TELL US?
-Some aligned residues may be highly conserved. For example, cysteine forms disulfide bridges.
-Some regions may have consistent patterns of insertions and deletions
FUNCTION,STRUCTURE AND EVOLUTION OF THE PROTEIN
Conserved motifs such as transmembrane domain and signal peptide
REVEAL CONSERVED RESIDUES OR MOTIFS
May have conserved secondary structure features
DETECT DISTANTLY RELATED MEMBERS
PHYLOGENETIC TREE
MSA : APPROACHES AND ITS APPLICATION
Zahir Haizat Bin Muhamad Zamani
(192155)
Shahrul Hasif Bin Zamal
(194257)
Ameera Aisyah Binti Azman
(193584)
Mastura Hanis Binti Baharom
(193816)
Anasuha Batrisyia Bilqis Binti Mat Saidi
(193670)
Nurulain Hasya Binti Azhar
(193668)
Nur Shazleen Amira Binti Sharol Nizam
(193669)
1. Choose homologous sequence to allign
2. Choose software that implements an appropriate objective scoring function
3. Choose appropriate parameters such as gap opening and gap extension penalties
MSA:HOW TO PERFORM?
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